Oligonucleotide synthesis is the chemical process by which nucleotides are specifically linked together to form the desired sequenced product.

Oligonucleotide scale-up synthesis is a major activity in the development of novel oligonucleotide-based drugs.

The Oligofastx project includes oligonucleotide scale-up activities using biosynthesis and solid-phase synthesis processes.

Oligonucleotide bisynthesis

Oligonucleotide biosynthesis production is based on the chemical synthesis of relatively short fragments of nucleic acid with a defined chemical structural sequence using biological processes and biological. The technique is extremely useful in current laboratory practice because it provides quick and cheap access to custom-made oligonucleotides with a desired sequence. While enzymes synthesise DNA and RNA in a 5′ to 3′ direction, oligonucleotide synthesis is carried out in the opposite direction, in the 3′ to 5′ direction.

Oligonucleotide biosyntesis is the most promising techique for a future green production of oligonucleotides.

Oligonucleotide solid-phase synthesis

Currently, the most feasible process for short oligonucleotide production, especially for oligonucleotides artificially modified, is implemented as a solid-phase synthesis using the phosphoramidite method and phosphoramidite building blocks derived from 2′-deoxynucleosides (dA, dC, dG and T), ribonucleosides (A, C, G, and U), or chemically modified nucleosides, such as ANB.

Oligofast Consortium has the optimal facilities and know-how for the development of scale-up of oligonucleotides used during the project or obtained from the research activities.

Oligonucleotide solid-phase synthesis

Oligonucleotide manufacturing by manufacturing is the chemical synthesis of relatively short fragments of nucleic acid with a defined chemical structural sequence using biological processes and biological . The technique is extremely useful in current laboratory practice because it provides quick and cheap access to custom-made oligonucleotides with a desired sequence. While enzymes synthesise DNA and RNA in a 5′ to 3′ direction, oligonucleotide synthesis is carried out in the opposite direction, in the 3′ to 5′ direction. Currently, the process is implemented as a solid-phase synthesis using the phosphoramidite method and phosphoramidite building blocks derived from 2′-deoxynucleosides (dA, dC, dG and T), ribonucleosides (A, C, G, and U), or chemically modified nucleosides, such as ANB.