RNA Bibliography: a selection of the most influential publications in the field of biotechnology

RNA Bibliography: a selection of the most influential publications in the field of biotechnology

In the field of biotechnology, RNA research is revolutionising the biomedical sector. From OLIGOFASTX we share an essential scientific bibliographic selection because the knowledge embodied in the publications is a reference for professionals.

There is much more, but the selection of RNA literature presented today is particularly relevant. Recall that RNA is a fundamental molecule in molecular biology, acting as an intermediary between DNA and proteins, and playing a key role in processes such as protein synthesis and gene regulation. In addition, RNA is also used in various biotechnological technologies, such as gene therapy and gene editing, a feature that we at OLIGOFASTX take as a starting point in our R&D work for the development of oligonucleotide-based therapies for rare diseases.

In this article, we will explore the main bibliographical references on RNA in the field of biotechnology and analyse some of the most influential publications in this field.

A selection of the most influential publications in the field of biotechnology: a look back at the last decades

Some of the most influential works in the field of biotechnology can be found in the detailed analysis of outstanding publications. First, the article “RNAi: Double-Stranded RNA Directs the ATP-Dependent Cleavage of mRNA at 21 to 23 Nucleotide Intervals” by Andrew Fire and Craig Mello, published in 1998, which describes the mechanism of RNA interference, a technique that has revolutionised the study of gene function. Second, David Bartel’s “MicroRNAs: Genomics, Biogenesis, Mechanism, and Function”, published in 2004, explores the role of microRNAs in gene regulation and their involvement in human disease. Finally, “The CRISPR-Cas9 System: Versatile Toolkit for Genome Engineering” by Jennifer Doudna and Emmanuelle Charpentier, published in 2012, describes a gene-editing technique that has transformed biotechnology and medicine. This work has had a significant impact on current scientific and technological development and demonstrates the importance of RNA for the advancement of biotechnology.

“RNAi: Double-Stranded RNA Directs the ATP-Dependent Cleavage of mRNA at 21 to 23 Nucleotide Intervals” – Andrew Fire y Craig Mello (1998)

One of the most outstanding works of this selection is the paper by Andrew Fire and Craig Mello, published in 1998, entitled “RNAi: Double-Stranded RNA Directs the ATP-Dependent Cleavage of mRNA at 21 to 23 Nucleotide Intervals”.

This paper describes how RNA interference (RNAi) can be used to silence specific genes in mammalian cells by introducing double-stranded RNA molecules that bind to messenger RNA (mRNA) and degrade it into fragments of 21-23 nucleotides. This work opened the door to a new way of controlling gene expression and has had a major impact on biomedical research and the pharmaceutical industry.

Our partner Sylentis – PharmaMar Group – is a Spanish company that is a pioneer in the R&D of these molecules as drugs.

“MicroRNAs: Genomics, Biogenesis, Mechanism, and Function” – David Bartel (2004)

Another influential publication is “MicroRNAs: Genomics, Biogenesis, Mechanism, and Function” by David Bartel, published in 2004.

In this paper, Bartel describes microRNAs, small RNA molecules that regulate gene expression post-transcriptionally. The article provides a detailed overview of the biogenesis and mechanism of action of microRNAs, as well as their role in various biological processes, such as development, cell differentiation and disease.

In addition, Bartel discusses the importance of microRNAs as potential therapeutic targets and as tools for disease diagnosis. This work has been fundamental to the understanding of microRNAs and their role in cell biology, and has driven the development of new therapies and diagnostics.

“The CRISPR-Cas9 System: Versatile Toolkit for Genome Engineering” – Jennifer Doudna y Emmanuelle Charpentier (2012)

We would also like to highlight “The CRISPR-Cas9 System: Versatile Toolkit for Genome Engineering” by Jennifer Doudna and Emmanuelle Charpentier (2012).

This publication describes the CRISPR-Cas9 system, a revolutionary tool for genome editing that has transformed biotechnology and medical research. This system allows researchers to edit and modify DNA more accurately and quickly than ever before.

Since its discovery by the Spaniard Francis Mojica, the CRISPR-Cas9 system has been used to treat genetic diseases, develop more resistant crops and create new animal models for research. This publication has been cited thousands of times and has been recognised with numerous awards and honours, including the 2015 Breakthrough Award.

The impact of the selected publications has been fundamental for the current technological and scientific development in the field of biotechnology. Andrew Fire and Craig Mello’s research in RNAi has enabled gene regulation through the targeted degradation of messenger RNA. On the other hand, David Bartel’s research on MicroRNAs has demonstrated their role in gene regulation and their potential in the diagnosis and treatment of diseases. Finally, Jennifer Doudna and Emmanuelle Charpentier’s research into the CRISPR-Cas9 system has revolutionised genetic engineering, enabling precise and efficient genome editing. These publications have been cited and used in numerous studies, demonstrating their influence on the advancement of biotechnology.


In addition to these, there are many other significant papers and publications in the field of RNA, some of which include:

Article: “The RNA revolution in biotechnology: principles and applications”.

Author: Rafael Tabarés

Source: Ribociencia Magazine

Link: https://www.ribociencia.com/la-revolucion-del-arn-en-biotecnologia/


Book: “RNA: World’s Versatile Key Molecule”.

Author: Catalina Cleland, Stefan Vogel, y Peter Schmechel

Source: World Scientific Publishing Co.

Link: https://www.worldscientific.com/worldscibooks/10.1142/11553


Article: “RNA technologies: the future of biotechnology”.

Author: Megan Chircop

Source: Pharmaceutical Patents Analyst

Link: https://www.future-science.com/doi/full/10.4155/PPA.14.3


Book: “RNA Interference: Methods for Plants and Animals”.

Authors: Nigel J. Mongomery, Philip D. Zamore

Source: Cold Spring Harbor Laboratory Press

Link: https://www.cshlpress.com/default.tpl?action=full&src=EN0003&category=ribo&search


Article: “RNA therapeutics: beyond RNA interference and antisense oligonucleotides”.

Authors: Nicole F. Steinmetz, Hiroyuki Takahashi, y Peixuan Guo

Source: Nature Reviews Drug Discovery

Link: https://www.nature.com/articles/nrd3923


Article: “RNA-Protein Interaction Detection using Crosslinking Immunoprecipitation (CLIP) Approaches”.

Authors: Mark B. Gerstein, Manolis Kellis

Source: Methods in Molecular Biology

Link: https://link.springer.com/protocol/10.1007/978-1-4939-0263-7_15

Article: “The role of RNA in biotechnology: The stories of RNA interference and RNA vaccines”. Author: Keck Graduate Institute

Article: “RNA interference: biology, mechanism, and applications”. Author: Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Source: Genes & Development Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC31290/

Book: “RNA Methodologies: Laboratory Guide for Isolation and Characterization”. Author: Robert E. Farrell Fuente: Academic Press Link: https://www.sciencedirect.com/book/9780121828158/rna-methodologies

Article: “RNA-editing-mediated recoding: A new mechanism for generating dysfunctional noncoding RNAs”. Author: Nishant S. Patel, Wenqian Hu, Saba Valadkhan Fuente: Seminars in Cell & Developmental Biology Link: https://www.sciencedirect.com/science/article/pii/S108495211630113X

Book: “RNA and the Regulation of Gene Expression: A Hidden Layer of Complexity”. Author: David L. Spector Fuente: Wiley Link: https://www.wiley.com/en-us/RNA+and+the+Regulation+of+Gene+Expression%3A+A+Hidden+Layer+of+Complexity-p-9781118542348

Article: “RNA-based therapeutics: current progress and future prospects”. Authors: Yin H, Kanasty RL, Eltoukhy AA, Vegas AJ, Dorkin JR, Anderson DG. Source: Chemical Reviews Link: https://pubs.acs.org/doi/10.1021/cr400107d

Article: “RNA as a tool and target of cellular physiology”. Author: Anthony A. Kossler, Ira S. Ozbolat, Binata Joddar, Jingwei Xie Fuente: Frontiers in Physiology Link: https://www.frontiersin.org/articles/10.3389/fphys.2017.00899/full